VNIRTM 2X Master Mix for PCR

Description:

VNIRTM PCR master mix is an optimized ready-to-use 2X formulation for PCR amplification of any genes. This master mix is useful for efficient amplification of GC rich, low copy number genes from various template types and can be used up to 40 -50 cycles. This master mix contains 2mM MgCl2 (in 1X final concentration).

Cat. No.: VNIR104

Highlights:

  • Extensively tested for reproducibility in PCR and RT-PCR.
  • Enzyme tested for amplification of genes up to 5kb and GC% >60.
  • Suitable for viral genes and synthetic vectors amplification as well.

Recommended reaction setup conditions:

Component
Volume
Final Concentration
VNIR 2X Master Mix10µl1X
Forward Primer – 10µM0.5µl500nM
Reverse Primer – 10µM0.5µl500nM
Template (cDNA/DNA)1-2µl10-100ng
VNIR NFWUp to 20µl 

*vortex briefly and spin down after addition of all components to ensure complete mixing

Amplification Conditions:

Step
Temperature
Time
Cycles
Enzyme Activation95°C3 min1
Denaturation95°C30 sec40-50
AnnealingPrimers Tm45 sec
Extension72°C1min/kb
Final Extension72°C10min1cycle
Cooling4°C 
Storage conditions:
-20°C
Best before:
24 months upon receiving
References:
Please let us know if you publish your research using VNIRTM 2X Master Mix. We can cite your reference here.