VNIRTM 2X Master Mix for PCR
Description:
VNIRTM PCR master mix is an optimized ready-to-use 2X formulation for PCR amplification of any genes. This master mix is useful for efficient amplification of GC rich, low copy number genes from various template types and can be used up to 40 -50 cycles. This master mix contains 2mM MgCl2 (in 1X final concentration).
Cat. No.: VNIR104
Highlights:
- Extensively tested for reproducibility in PCR and RT-PCR.
- Enzyme tested for amplification of genes up to 5kb and GC% >60.
- Suitable for viral genes and synthetic vectors amplification as well.
Recommended reaction setup conditions:
Component | Volume | Final Concentration |
| VNIR 2X Master Mix | 10µl | 1X |
| Forward Primer – 10µM | 0.5µl | 500nM |
| Reverse Primer – 10µM | 0.5µl | 500nM |
| Template (cDNA/DNA) | 1-2µl | 10-100ng |
| VNIR NFW | Up to 20µl |
*vortex briefly and spin down after addition of all components to ensure complete mixing
Amplification Conditions:
Step | Temperature | Time | Cycles |
| Enzyme Activation | 95°C | 3 min | 1 |
| Denaturation | 95°C | 30 sec | 40-50 |
| Annealing | Primers Tm | 45 sec | |
| Extension | 72°C | 1min/kb | |
| Final Extension | 72°C | 10min | 1cycle |
| Cooling | 4°C | ∞ |
Storage conditions: | -20°C |
Best before: | 24 months upon receiving |
References: | Please let us know if you publish your research using VNIRTM 2X Master Mix. We can cite your reference here. |