VNIRTM Green 2X Master Mix (no ROX) for Real time PCR

Description:

VNIRTM Green qPCR Master mix is an optimized ready-to-use 2X master mix for real-time PCR assays (Use SyBr Green channel in all thermal cyclers). This master mix is useful for efficient amplification of GC rich, low copy number genes from various template types and can be used up to 60 cycles. This master mix contains 2.5mM MgCl2 (in 1X final concentration).

Cat. No.: VNIR102

Highlights:

  • Low non-specific and background fluorescence.
  • GC rich and low copy number genes quantification.
  • Compatible for most of the target genes.

Recommended reaction setup conditions:

Component
Volume
Final Concentration
VNIR Green 2X Master Mix10µL1X
Forward Primer – 10µM0.5µL500nM
Reverse Primer – 10µM0.5µL500nM
TemplateVariable (2-3µL) 
VNIR NFWUp to 20µL 

*vortex briefly and spin down after addition of all components to ensure complete mixing

Quality Control:

Assay
Results
Amplification efficiency100%
RTqPCR reproducibilityPassed
ds Endodeoxyribonuclease activityPassed
ss Exodeoxyribonuclease activityPassed
Self-priming activityNot observed

Amplification Conditions:

StepTemperatureTimeCycles
Enzyme Activation95°C3 min1
Denaturation95°C20 sec40-50
Annealing/ElongationPrimers Tm45 sec
Melt Profile50°C-95°C0.5°C/30sec
Cooling8°C
Storage conditions:
-20°C (protect from light)
References:
Please let us know if you publish your research using VNIRTM Green 2X Master Mix. We can cite your reference here.
Note:
This formulation doesn’t contain ROX.

4