VNIRTM HotStart 2X master mix for PCR

Description:

VNIRTM HotStart master mix is an optimized ready-to-use 2X master mix for PCR amplification of any genes. To prevent non-specific amplification antibody trapped HotStart DNA polymerase is employed which needs initial activation at 95°C for 6min. This master mix is useful for efficient amplification of GC rich, low copy number genes from various template types and can be used up to 50 cycles. This master mix contains 1.5 mM MgCl2 (1X final concentration).

Cat. No.: VNIR103

Highlights:

  • Extensively tested for reproducibility in PCR and RT-PCR.
  • Enzyme tested for amplification of genes up to 8kb and GC% >60.
  • Suitable for viral genes amplification as well.

Quality Control:

Assay
Results
Amplification efficiency>105 fold
qPCR reproducibilityPassed
ds Endodeoxyribonuclease activityPassed
ss Exodeoxyribonuclease activityPassed
Self-priming activityNot observed
Storage conditions:
- 20°C

Amplification conditions:

Activation
Denaturation
Annealing
Extension
Final Extension
Cooling
95°C95°CPrimer Tm72°C72°C4°C
6 min30 sec45 sec1 min/kb10 min
1 cycle35-50 cycles1 cycle