VNIR^TM Green 2X One-step RT-qPCR Master Mix (ROX)

All Products Molecular Biology Products VNIR 115

Description

VNIR^TM Green 2X One-step RT-qPCR Master Mix is based on quantitative reverse transcription PCR (RT-qPCR) that uses RNA as the starting material. It offers a convenient master mix to convert RNA to DNA and quantify in a one-step reaction. The single-tube 2X formulation contains MMLV reverse transcriptase, dNTPs, VNIR Green (equivalent to Sybr® Green), Taq DNA polymerase, reaction buffer, MgCl₂, KCl, and PCR enhancer/stabilizer facilitating easy reaction set up. The user needs only to add the template, the primer set and water to the master mix to set up the RT-PCR reaction. This master mix is useful for efficient amplification of GC rich, low copy number genes from various template types and can be used up to 80 cycles. This master mix contains 2.5mM MgCl₂ (1X final concentration).

Cat. No.: VNIR115

Quality Control

Assay	Results
Amplification efficiency	>105 fold
qPCR reproducibility	Passed
ds Endodeoxyribonuclease activity	Not observed
ss Exodeoxyribonuclease activity	Not observed
Self-priming activity	Not observed

Recommended reaction setup conditions

Component	Volume	Final Concentration
VNIR Green 2X One-step RT-qPCR Master Mix	10µl	1X
Forward Primer – 10µM	0.5µl	500nM
Reverse Primer – 10µM	0.5µl	500nM
RNA Template	Variable	50-400ng
PCR grade water	Variable	–
Total Volume per reaction	20 µl	–

*vortex briefly and spin down after addition of all components to ensure complete mixing

Amplification Conditions

Step	Temperature	Time	Cycles
RT	42°C	5-10 min	1
Enzyme Activation	95°C	3 min	35-45
Denaturation	95°C	30 sec
Annealing, extension and read fluorescence	Primers Tm	30 sec

Storage conditions	-20°C (protect from light)
Best before	24 months upon receiving
References	Please let us know if you publish your research using VNIRTM Green 2X One-step RT-qPCR Master Mix. We can cite your reference here.
Note	This formulation doesn’t contain ROX.